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功能代谢组学揭示黄芪多糖通过2-羟基丁酸改善肥胖小鼠的脂质代谢 Article
李冰冰, 洪颖, 顾彧, 叶圣洁, 胡凯莉, 姚建, 丁侃, 赵爱华, 贾伟, 李后开
《工程（英文）》 2022年 第9卷 第2期 页码 111-122 doi: 10.1016/j.eng.2020.05.023
富含sn-2 DHA脂质对大脑的益处及其酶法合成综述 Review
金俊, 金青哲, 王兴国, Casimir C. Akoh
《工程（英文）》 2020年 第6卷 第4期 页码 424-431 doi: 10.1016/j.eng.2020.02.009
关键词： DHA 和 sn-2 DHA 单甘酯 大脑 肠-脑轴 结构脂质
代谢组扩展生物学的“旁中心法则”——对理解基因组学-糖组学-代谢组学-表观基因组学互作的意义
Albert Stuart Reece
《工程（英文）》 2023年 第26卷 第7期 页码 16-16 doi: 10.1016/j.eng.2022.07.011
The central dogma of biology holds that the transcription of DNA into RNA and the translation of RNA into proteins forms the primary axis of biological activity [1]. Following major advances in the description of the complex glycan and lipid chains that are added onto these basic building blocks, the glycome and lipidome have recently been added to this doctrine as an exciting new extension named the ‘‘paracentral dogma” [2]. However, it has been pointed out that biological systems can include many layers, which are described in modern omics technology platforms relating to both cell-intrinsic and cell-extrinsic layers of control, including metabolomic, microbiomic, immunological, epigenomic, epitranscriptomic, proteomic and phosphoproteomic layers [3].
It is well known that stem and progenitor cells have a metabolism that is based on glycolysis and glutaminolysis [4]. Although this provides less energy to the cell than oxidative phosphorylation, it suffices for these cells’ needs, since such cells are generally relatively quiescent and normally suppress energy-intensive processes such as genome duplication and transcription. Moreover, it has been shown that the high intracellular lactate levels involved in such states not only inhibits the key gatekeeper enzymes of oxidative phosphorylation (i.e., pyruvate dehydrogenase and carnitine palmitoyl acyltransferase) but also actually covalently modifies them by lactylation in order to maintain this inhibited metabolic–epigenomic state [5]. In addition, intermediate metabolism and nutrients are the source of the very extensive library of post-translational modifications to DNA, RNA, and proteins, as well as supplying cellular energy for many of the required reactions. Hence, the metabolic state locks in and reinforces the epigenomic state, and the metabolome and epigenome thereby play mutually reinforcing roles. This self-reinforcing coordination explains why it is so difficult to generate induced pluripotent cells and is a contributory explanation for why the described protocols typically have such low cellular yields.
These concepts become even more important when it is considered that cancer cells are de-differentiated, similarly rely on glycolysis and glutaminolysis, and are similarly metabolically–epigenomically–genomically synchronized. The disruption of this metabolic system is a key focus of mechanistic cancer research.
These important considerations imply that the descriptive and predictive power of the newly described ‘‘paracentral dogma” of biology may be usefully and meaningfully extended by including the metabolome, along with the genome, transcriptome, proteome, glycome, and lipidome, to describe cell-intrinsic regulation—not only in terms of another omics analytical layer but also as a fully predictive and interactive partner in the symphonic-like multilayer coordination that evidently comprises cellular regulatory layering.
杜金名
《中国工程科学》 2005年 第7卷 第1期 页码 65-68
假单胞菌环状脂肽Medpeptin的生物合成及其调控植物免疫的作用机制 Article
谷医林, 李俊州, 李彦, 从珅, 王静, 马毅楠, 魏海雷
《工程（英文）》 2023年 第28卷 第9期 页码 153-165 doi: 10.1016/j.eng.2023.05.016
环状脂肽（cyclic lipopeptides, CLP）是一类由细菌产生的多功能次生代谢产物，也是一类重要的植物免疫激发子。假单胞菌环状脂肽在结构和生物活性上极其复杂和多样化。但是，目前对环状脂肽的结构及其与植物互作中的功能认识仍比较有限。本研究从地中海假单胞菌中鉴定了一种由22个氨基酸组成的新型的环状脂肽medpeptin，该脂肽由一个非核糖体肽合成酶基因簇合成并受群体感应系统调节。
关键词： 假单胞菌 环状脂肽 细胞死亡 富含异亮氨酸的延伸蛋白 类受体激酶
中医方证代谢组学——中药效应评价的有效途径 Review
张爱华, 孙晖, 闫广利, 韩莹, 赵琦琦, 王喜军
《工程（英文）》 2019年 第5卷 第1期 页码 60-68 doi: 10.1016/j.eng.2018.11.008
韩 达,吴天植,辛显双,王绍荣,占忠亮
《中国工程科学》 2013年 第15卷 第2期 页码 66-71
谢富丞,王诚,毛宗强
《中国工程科学》 2013年 第15卷 第2期 页码 72-76
范兴祥,杨晶晶,黄铭,汪云华,李柏榆
《中国工程科学》 2007年 第9卷 第11期 页码 200-203
利用微波技术快速测定镁质硅酸镍矿的水分，并对不同含水量的镁质硅酸镍矿对检测结果的影响 进行了分析和讨论；对微波检测装置、特性及其操作方法做了说明
通过定量分析植物初级代谢产物和酶代谢库发现除草剂新作用位点 Perspective
Franck E. Dayan, Stephen O. Duke
《工程（英文）》 2020年 第6卷 第5期 页码 509-514 doi: 10.1016/j.eng.2020.03.004
苗德海,莫阳春,王伟,陈涛
《中国工程科学》 2009年 第11卷 第12期 页码 41-46
一种基于Caco-2细胞单层膜的筛选抵抗特定代谢性疾病益生菌的模型 Article
刘阳, 彭江, 朱诗雅, 于雷雷, 田丰伟, 赵建新, 张灏, 陈卫, 翟齐啸
《工程（英文）》 2023年 第25卷 第6期 页码 222-233 doi: 10.1016/j.eng.2022.02.014
最近的研究揭示了益生菌在缓解与肠道屏障功能障碍相关的代谢疾病方面的潜力。然而，目前还没有一种能高效筛选抵抗特定代谢疾病益生菌菌株的模型。本研究建立了一种基于Caco-2细胞单层膜模型的体外方法，用于高效筛选可以抵抗特定代谢疾病（如骨质疏松症和慢性酒精性肝病）的益生菌。
普列克底物蛋白同源物样结构域家族A成员1蛋白——导致代谢疾病的多方面细胞存活因素 Review
Tamana Yousof, Jae Hyun Byun, Jack Chen, Richard C. Austin
《工程（英文）》 2023年 第20卷 第1期 页码 9-18 doi: 10.1016/j.eng.2022.05.014
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